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RS-G4 Upright Research Confocal Microscope

Speed, Flexibility, and Precision in an
Easy-To-Use Design

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The upright RS-G4 research confocal microscope features a new strip mosaic image capture process coupled with its proprietary image-stitching algorithm to create cellular-level images with unprecedented down-to-the-pixel accuracy and perfect homogeneity. The process generates large-scale mosaics that you can use to quickly locate a data collection area on a large specimen, such as a whole brain section.

APPLICATIONS

Neuroscience: The high-speed strip mosaic feature of the RS-G4 can scan large areas of whole brain sections in just a few minutes. Full correlation is available to interact from the macro view to zoom into the desired micro cellular detail for various fluorescent stained image views. The mosaics are processed using an image-stitching algorithm to display perfectly aligned images with seamless frame-to-frame clarity.

Developmental Biology: Observing animal development has traditionally required taken several instruments to record growth, from high-power microscopes to larger Field of View (FOV) stereo microscopes. The RS-G4 lets you complete the same tasks with a single instrument. It can record confocal imaging at all stages of development, with many fluorophores highlighting cellular development from sub-micron resolution to complete FOVs of 30 x 30 mm and larger, covering micro to macro imaging.

In addition to visible wavelengths, the RS-G4 provides an infrared laser (785nm or 830nm) for in-vivo sample imaging with a penetration depth of up to 250 microns (depending on cellular density). The ability to look inside your juvenile or adult model adds new information to your research.

Pathology: The RS-G4 supports both confocal reflectance microscopy (CRM) and confocal fluorescence microscopy (CFM) for the ex vivo applications involved in reviewing images of tissue.

Translational Research: The near 25 frames-per-second image capture speed and extensive functionality of the RS-G4 bring confocal microscopy to a wide range of new research areas.


SPECIFICATIONS

Resolution

  • Horizontal Optical Resolution: 0.5 µm
  • Vertical Optical Resolution: 0.8 µm
  • Displayed Image Resolution: 1024 x 1024 pixels

Field

  • Single Frame FOV: 400 µm x 400 µm standard objective
  • Mapped Field: 120 x 80 mm
  • Magnification: Olympus 40x/1.3 oil objective (Standard) Optional 20x, 40x dry/water/oil, 60x water/oil
  • Image Digitization: 8 displayed; 16 bit TIFF stored
  • Frame Rate: Variable (5-25 fps)
  • Operating Wavelengths include: 405 / 488 / 638 / 561 / 785 nm

Environmental

  • Optical Operating Power: Class 3B
  • Operating Temperature: 55°F to 85°F (13°C to 30°C)
  • Power Source: 110-230 VAC 50-60 Hz
  • Operating Humidity: Non-condensing

Physical

  • Dimensions: L 18.25 x W 15.5 x H 16
  • Scan Head Only: L 6.32 x W 4.3 x H 11.55
  • Monitor: 27", 2560 x 1440 pixels (WQHD) 16:9

Click to enlarge:


Mouse Brain
Image courtesy of Boston Children's Hospital
D. Ehlinger and K. Commons

HIGHLIGHTS

  • Fast, large-scale mosaic creation up to 120 x 80 mm
  • To-the-pixel image accuracy
  • Automated simultaneous and sequential reflectance and fluorescence imaging
  • Multi-dimensional imaging
  • Intuitive microscope control
  • Experiment acquisition setup for automated collection of snapshots, time, X-Y-Z, multi-stage points, wavelength)
  • Supports standard dry, and oil and water immersion objectives
  • Windows® based image storage structure

Fast, Accurate Large-Scale Mosaics

This upright research confocal microscope quickly creates the large-scale mosaics you need to locate and analyze target areas on your specimens. The mosaics are created with a multi-dimensional imaging system driven by high-speed strip mapping software that launches as the first strip is imaged. As each subsequent strip is imaged, the software performs Seam Carving that stitches the fields together with perfect alignment. You get an image with cellular level, down-to-the-pixel accuracy that delivers perfect homogeneity, generated with virtually no delay. Slashing image acquisition time with high-accuracy strip mapping gives you a superior source for analysis, and dramatically reduces the time required to complete experiments. Customers have reported reducing large area imaging times from two weeks to less than an hour. Faster workflow cycles valuable free time and create a better user experience.

Download RS-G4 Press Release

Download Brochure

Download Technical Note